Coding

Part:BBa_K2382001:Design

Designed by: SHAO-CHI LO   Group: iGEM17_CSMU_NCHU_Taiwan   (2017-10-26)


MSMEG_5998

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 428
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It has not been coden optimized for E. Coli BL21, which however seems to have no effect on protein expression. Otherwise, we remove the stop coden of NC_008596.1 The sequence of this part is from Australian.(While the sequence in BBa_K2382006 is modified by ourselves.)

Source

Mycobacterium smegmatis str. MC2 155 https://www.ncbi.nlm.nih.gov/nuccore/118467340/